Products
C·Prep Urine Kit
The C·Prep urine kit is is an RNA extraction kit optimized for human urine. The kit allows for remote collection of samples. The one-step process simply requires adding fresh urine to a mixture and stabilizes RNA for up to 10 days. The two-step collection process involves adding the fresh urine to a mixture, placing the mixture on a magnetic stand, pouring out excess lysis buffer after a few minutes, and then adding ethanol. The two-step collection process can stabilize a sample at room temperature for 25+ days. See the performance section for additional information.
Available Options:
| Purpose | RNA isolation/purification from urine |
|---|---|
| Format and Technology | Magnetic Beads; Carbon Technology |
| Processing Time | 15 min |
| Type of RNA Purified | Total RNA (includes ribosomal RNA, mRNA, and small RNAs) |
| Application | Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing |
| Processing | Manual or automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation to release RNA. |
| Main Sample Type | Urine Samples. |
| Elution Volume | 30-100 µl |
| Sample Size | 100 ul is the standard input volume and reagent consumption scales with the sample volume. Lower volumes may be used and the reagent use scales accordingly meaning that if lower volumes are used the kit will be good for more than the listed number of reactions and vice versa. |
| Kit Storage | Room Temperature |
The C·Prep Urine Kit is intended for both purification and stabilization of RNA from urine samples. The kit includes both a one-step collection method which stabilizes RNA in freshly collected urine for 10 days and a two-step collection method which stabilizes RNA in freshly collected urine for up to 25 days. Provided below are expression levels of various housekeeping genes using the two collection methods at different time points.
Li X, Frazier JA, Spahiu E, McPherson M, Miller RA. Muscle-dependent regulation of adipose tissue function in long-lived growth hormone-mutant mice. Aging (Albany NY). 2020;12(10):8766‐8789. doi:10.18632/aging.103380
Jones, L. B. et al. Effects of Pseudomonas aeruginosa on microglial-derived extracellular vesicle biogenesis and composition. Pathogens 8, 297 (2019). doi:10.3390/pathogens8040297
Shah, S., Brock, E. J., Jackson, R. M., Ji, K., Boerner, J. L., Sloane, B. F., & Mattingly, R. R. (2018). Downregulation of Rap1Gap: A Switch from DCIS to Invasive Breast Carcinoma via ERK/MAPK Activation. Neoplasia (New York, N.Y.), 20(9), 951–963. doi:10.1016/j.neo.2018.07.002
- “The main drawback from my current RNA isolation protocol is the numerous centrifugation steps and the relatively low volume of RNA yield in the end. Life Magnetics provides a higher yield of RNA from samples and eliminates the use of centrifugation steps.”
– Kendra Royston, Ph.D. at University of Chicago - “Life Magnetics has a new technology, which is worth trying and their biggest advantage is skipping DNase step.”
– Quan Taihao, Ph.D. and Qin Zhaoping at University of Michigan, Dermatology Department - “It is easy to use and gives high quality and a greater yield at a much-reduced price. Also, the stability of RNA with the beads at room temperature before the final extraction step is a big plus.”
– Sabita Saldanha, Ph.D. at Alabama State University - “This system is significantly easier than Trizol because it can be done on the bench top, rather than a fume hood. It is of comparable ease to Qiagen systems. A potential advantage that this kit has over a Qiagen system is that it can be scaled more easily.”
– Joe Endicott, Ph.D. at University of Michigan, School of Medicine Ann Arbor - “With Life Magnetics Carbon-Based RNA extraction kit, it was nice to switch things up and try a different protocol. The extraction method generated great RNA purity and yield.”
– Kayla Lewis, Ph.D. at the University of Alabama at Birmingham - “When I used the Life Magnetics IsoMag Carbon Based RNA purification kit, I was surprised how easy protocol was to follow and how much more RNA was extracted. The major advantage of this kit over other commercial kits is high RNA yield with no additional DNase cleanup steps.”
– Seema Shah, Ph.D. at Wayne State University
Safety data sheets for all materials included in kit:
- RPS A Buffer
- Neutralization Buffer
- Binding Buffer
- Carbon magnetic particles
- RPS Wash buffer
- Wash buffer
- Elution Buffer