The C·Prep urine kit is is an RNA extraction kit optimized for human urine. The kit allows for remote collection of samples. The one-step process simply requires adding fresh urine to a mixture and stabilizes RNA for up to 10 days. The two-step collection process involves adding the fresh urine to a mixture, placing the mixture on a magnetic stand, pouring out excess lysis buffer after a few minutes, and then adding ethanol. The two-step collection process can stabilize a sample at room temperature for 25+ days. See the performance section for additional information.
|Purpose||RNA isolation/purification from urine|
|Format and Technology||Magnetic Beads; Carbon Technology|
|Processing Time||15 min|
|Type of RNA Purified||Total RNA (includes ribosomal RNA, mRNA, and small RNAs)|
|Application||Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing|
|Processing||Manual or automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation to release RNA.|
|Main Sample Type||Urine Samples.|
|Elution Volume||30-100 µl|
|Sample Size||100 ul is the standard input volume and reagent consumption scales with the sample volume. Lower volumes may be used and the reagent use scales accordingly meaning that if lower volumes are used the kit will be good for more than the listed number of reactions and vice versa.|
|Kit Storage||Room Temperature|
The C·Prep Urine Kit is intended for both purification and stabilization of RNA from urine samples. The kit includes both a one-step collection method which stabilizes RNA in freshly collected urine for 10 days and a two-step collection method which stabilizes RNA in freshly collected urine for up to 25 days. Provided below are expression levels of various housekeeping genes using the two collection methods at different time points.
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