C·Prep viral transport media (VTM) and saliva kit is optimized for use with these sample types. The kit can use either an acid-guanidinium-phenol lysis buffer purchased externally or an acid-guanidinium and proteinase K lysis buffer provided with the kit.
Currently, due to shortages in proteinase K, we recommend using an acid-guanidinium-phenol lysis buffer.
|Purpose||RNA isolation/purification from viral transport media (VTM) and saliva|
|Format and Technology||Magnetic Beads; Carbon Technology|
|Processing Time||15 min|
|Type of RNA Purified||Total RNA (includes ribosomal RNA, mRNA, and small RNAs)|
|Application||Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing|
|Processing||Manual or automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation to release RNA.|
|Main Sample Type||Viral transport media and saliva samples|
|Elution Volume||30-100 µl|
|Sample Size||100 ul is the standard input volume and reagent consumption scales with the sample volume. Lower volumes may be used and the reagent use scales accordingly meaning that if lower volumes are used the kit will be good for more than the listed number of reactions and vice versa.|
|Kit Storage||Room Temperature|
Carbon prep magnetic beads can be part of a successful testing platform. Carbon prep performs similarly to silica beads in simple extraction applications. The major advantage over silica technologies is that carbon has virtually no DNA contamination, which is important for saliva testing and carbon can help stabilize RNA for transport in remote and at-home testing applications.
This field is rapidly evolving, for the most up-to-date information, contact us at firstname.lastname@example.org
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