Products
C·Prep Viral RNA
C·Prep viral transport media (VTM) and saliva kit is optimized for use with these sample types. The kit can use either an acid-guanidinium-phenol lysis buffer purchased externally or an acid-guanidinium and proteinase K lysis buffer provided with the kit.
Currently, due to shortages in proteinase K, we recommend using an acid-guanidinium-phenol lysis buffer.
Available Options:
| Purpose | RNA isolation/purification from viral transport media (VTM) and saliva |
|---|---|
| Format and Technology | Magnetic Beads; Carbon Technology |
| Processing Time | 15 min |
| Type of RNA Purified | Total RNA (includes ribosomal RNA, mRNA, and small RNAs) |
| Application | Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing |
| Processing | Manual or automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation to release RNA. |
| Main Sample Type | Viral transport media and saliva samples |
| Elution Volume | 30-100 µl |
| Sample Size | 100 ul is the standard input volume and reagent consumption scales with the sample volume. Lower volumes may be used and the reagent use scales accordingly meaning that if lower volumes are used the kit will be good for more than the listed number of reactions and vice versa. |
| Kit Storage | Room Temperature |
- Viral kit quick protocol for viral transport media and saliva
- Video Walk-Through For RNA Extraction
- Rebinding Assay – RNA Rebinding/Cleanup
Carbon prep magnetic beads can be part of a successful testing platform. Carbon prep performs similarly to silica beads in simple extraction applications. The major advantage over silica technologies is that carbon has virtually no DNA contamination, which is important for saliva testing and carbon can help stabilize RNA for transport in remote and at-home testing applications.
This field is rapidly evolving, for the most up-to-date information, contact us at info@magnetics.life
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Shah, S., Brock, E. J., Jackson, R. M., Ji, K., Boerner, J. L., Sloane, B. F., & Mattingly, R. R. (2018). Downregulation of Rap1Gap: A Switch from DCIS to Invasive Breast Carcinoma via ERK/MAPK Activation. Neoplasia (New York, N.Y.), 20(9), 951–963. doi:10.1016/j.neo.2018.07.002
- “The main drawback from my current RNA isolation protocol is the numerous centrifugation steps and the relatively low volume of RNA yield in the end. Life Magnetics provides a higher yield of RNA from samples and eliminates the use of centrifugation steps.”
– Kendra Royston, Ph.D. at University of Chicago - “Life Magnetics has a new technology, which is worth trying and their biggest advantage is skipping DNase step.”
– Quan Taihao, Ph.D. and Qin Zhaoping at University of Michigan, Dermatology Department - “It is easy to use and gives high quality and a greater yield at a much-reduced price. Also, the stability of RNA with the beads at room temperature before the final extraction step is a big plus.”
– Sabita Saldanha, Ph.D. at Alabama State University - “This system is significantly easier than Trizol because it can be done on the bench top, rather than a fume hood. It is of comparable ease to Qiagen systems. A potential advantage that this kit has over a Qiagen system is that it can be scaled more easily.”
– Joe Endicott, Ph.D. at University of Michigan, School of Medicine Ann Arbor - “With Life Magnetics Carbon-Based RNA extraction kit, it was nice to switch things up and try a different protocol. The extraction method generated great RNA purity and yield.”
– Kayla Lewis, Ph.D. at the University of Alabama at Birmingham - “When I used the Life Magnetics IsoMag Carbon Based RNA purification kit, I was surprised how easy protocol was to follow and how much more RNA was extracted. The major advantage of this kit over other commercial kits is high RNA yield with no additional DNase cleanup steps.”
– Seema Shah, Ph.D. at Wayne State University
Safety data sheets for all materials included in kit: