CarbonMag is a disruptive new RNA purification technology which uses carbon surfaces, rather than silica surfaces, to isolate RNA from a mixture. This kit and protocol are for obtaining RNA from blood samples.
Note: This kit requires reagents and equipment not sold here. It requires Proteinase K and an OmniLyse Homogenizer.
|Purpose||RNA isolation/purification from blood|
|Format and Technology||Magnetic Beads; Carbon Technology, requires mechanical method for cell lysis|
|Processing Time||15 min|
|Type of RNA Purified||Total RNA (includes ribosomal RNA, mRNA, and small RNAs)|
|Application||Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing|
|Processing||Manual or automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation to release RNA.|
|Main Sample Type||Blood samples. All C·Prep kits can be reconfigured into other C·Prep kits with minor changes to the buffers see the kit contents document under protocols for details.|
|Elution Volume||30-100 µl|
|Sample Size||100 ul is the standard input volume and reagent consumption scales with the sample volume. Lower volumes may be used and the reagent use scales accordingly meaning that if lower volumes are used the kit will be good for more than the listed number of reactions and vice versa.|
|Kit Storage||Room Temperature|
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