Carbon Prep for Wastewater RNA extraction
Dramatically improve RNA extraction from dilute solutions like COVID-19 detection in wastewater using our carbon prep RNA isolation kits.
About the process
The most advanced technology for detecting trace RNA analytes in dilute solutions
Minimal Equipment Required
Extract RNA from up to 1000 ml with only a magnetic stand and heating block.
Simple and Rapid Method
Complete extraction in just over 1 hour as compared to more than 1 day with precipitation methods.
High Throughput
Automated washing, elution, and plating for PCR on an Opentrons liquid handler with up to 96 samples at a time for less than $20,000.
Method
Carbon prep allows you to directly extract RNA from dilute solutions
No need for tedious precipitation of solids before extraction and no need for centrifuges or a cold room. Carbon technology allows you to pull RNA directly from the sample for faster sample preparation and superior results.
PEG Technique
1. Mix
20 mins
2. Spin
2 - 16 hrs
3. Centrifuge
1 hr
4. Resuspend
20 mins
5. Extract
1 hr
Total Time: 4.5 - 18.5 hrs
Life Magnetics Technique
1. Mix/Head
30 mins
2. Bind
10 mins
3. Separate
10 mins
4. Wash/Resuspend
20 mins
5. Elute
10 mins
Total Time: 80 mins
Example Data
Sampling Wastewater for SARS-CoV-2/COVID-19
99%
RNA Recovery
Wastewater spiked with 20,000 copies of Phi 6 virus showed 99% recovery from 100 ml of wastewater.
100/ml
RNA Recovery
60 min process time. Go from raw sample to PCR-ready RNA in 1 hour.
What's Included
All Required Chemicals for RNA Isolation
Includes all required chemicals for RNA isolation, PCR reagents should be purchased separately
Equipment Needed
Hot Water Bath
Samples will need to be heated to 95C for 30 min.
Magnetic Stand
Magnetic stand sold by Life Magnetics is compatible with a variety of 250 ml sample bottles including most common environmental collection bottles and common laboratory glass bottles.