C·Prep Wastewater Kit
The C·Prep wastewater kit is is an RNA extraction kit optimized for wastewater. The kit allows for remote collection of samples. The one-step process simply requires adding Life Magnetics’ beads to the wastewater and incubating for 4 hours at room temperature. This stabilizes RNA and DNA onto the beads which is then extracted following our standard protocol. See the performance section for additional information.
Interested in learning how Life Magnetics simplifies wastewater testing to more accurately monitor emerging diseases? Read our blog post here.
|RNA/DNA isolation/purification from wastewater
|Format and Technology
|Magnetic Beads; Carbon Technology
|15 minutes hands on time. 30 min to 2h total process time depending on input volume and water quality
|Type of RNA Purified
|Total RNA (includes ribosomal RNA, mRNA, and small RNAs) and DNA (includes genomic DNA and plasmid DNA)
|Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing
|Manual or semi-automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation and heat to release RNA.
|Main Sample Type
|0.1 ml to 1000 ml. Typical input is 10 or 20 ml.
The C·Prep Wastewater Kit is intended for both purification and stabilization of RNA from wastewater samples. The kit includes a one-step collection method which stabilizes RNA in freshly collected wastewater up to currently 50mL. For detailed information regarding performance click here.
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- “The main drawback from my current RNA isolation protocol is the numerous centrifugation steps and the relatively low volume of RNA yield in the end. Life Magnetics provides a higher yield of RNA from samples and eliminates the use of centrifugation steps.”
– Kendra Royston, Ph.D. at University of Chicago
- “Life Magnetics has a new technology, which is worth trying and their biggest advantage is skipping DNase step.”
– Quan Taihao, Ph.D. and Qin Zhaoping at University of Michigan, Dermatology Department
- “It is easy to use and gives high quality and a greater yield at a much-reduced price. Also, the stability of RNA with the beads at room temperature before the final extraction step is a big plus.”
– Sabita Saldanha, Ph.D. at Alabama State University
- “This system is significantly easier than Trizol because it can be done on the bench top, rather than a fume hood. It is of comparable ease to Qiagen systems. A potential advantage that this kit has over a Qiagen system is that it can be scaled more easily.”
– Joe Endicott, Ph.D. at University of Michigan, School of Medicine Ann Arbor
- “With Life Magnetics Carbon-Based RNA extraction kit, it was nice to switch things up and try a different protocol. The extraction method generated great RNA purity and yield.”
– Kayla Lewis, Ph.D. at the University of Alabama at Birmingham
- “When I used the Life Magnetics IsoMag Carbon Based RNA purification kit, I was surprised how easy protocol was to follow and how much more RNA was extracted. The major advantage of this kit over other commercial kits is high RNA yield with no additional DNase cleanup steps.”
– Seema Shah, Ph.D. at Wayne State University