The C·Prep wastewater kit is is an RNA extraction kit optimized for wastewater. The kit allows for remote collection of samples. The one-step process simply requires adding Life Magnetics’ beads to the wastewater with sarkosyl and TCEP and heating at 95C. This stabilizes RNA onto the beads which is then extracted following our standard protocol. See the performance section for additional information.
|Purpose||RNA isolation/purification from wastewater|
|Format and Technology||Magnetic Beads; Carbon Technology|
|Processing Time||2 Hours|
|Type of RNA Purified||Total RNA (includes ribosomal RNA, mRNA, and small RNAs)|
|Application||Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing|
|Processing||Manual or semi-automated. Manual protocols use a water bath to release RNA, while automated protocols use robotic agitation and heat to release RNA.|
|Main Sample Type||Wastewater Samples.|
|Elution Volume||30-100 µl|
|Sample Size||100 ul is the standard input volume and reagent consumption scales with the sample volume. Lower volumes may be used and the reagent use scales accordingly meaning that if lower volumes are used the kit will be good for more than the listed number of reactions and vice versa.|
|Kit Storage||Room Temperature|
The C·Prep Wastewater Kit is intended for both purification and stabilization of RNA from wastewater samples. The kit includes a one-step collection method which stabilizes RNA in freshly collected wastewater up to currently 50mL. For detailed information regarding performance click here.
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