$180.00 – $800.00
CarbonPrep for cell cultures is a ready-to-use kit which comes with buffers formulated for use with cultured cells.
This kit is shipped with buffers pre-optimized for cell cultures, which are usually washed with phosphate buffered saline (PBS) and pelleted. The buffers and protocol included with this product have been optimized for use with samples that have a high PBS and salt content.
|Processing Time||15 min|
|Sample Sources||Cultured cells|
|Application||Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, next-generation sequencing|
|RNA Purity||A260/A280 > 1.95
A260/A230 > 1.95
|RNA Type||Total RNA: miRNA and mRNA|
|Elution Volume||30-100 µl|
|Sample Size||1-3 million cells is the standard sample size but the protocol can be scaled to larger or smaller sizes.
The kit will last for more then 50 preps with smaller sample sizes and fewer than 50 preps with larger sample sizes.
|Kit Storage||Neutralization buffer should be stored in the fridge, lysis buffer should be stored out of the sun.|
Shah, S., Brock, E. J., Jackson, R. M., Ji, K., Boerner, J. L., Sloane, B. F., & Mattingly, R. R. (2018). Downregulation of Rap1Gap: A Switch from DCIS to Invasive Breast Carcinoma via ERK/MAPK Activation. Neoplasia (New York, N.Y.), 20(9), 951–963.