CarbonPrep Cells Kit

• Cultured cells quick start protocol

Shah, S., Brock, E. J., Jackson, R. M., Ji, K., Boerner, J. L., Sloane, B. F., & Mattingly, R. R. (2018). Downregulation of Rap1Gap: A Switch from DCIS to Invasive Breast Carcinoma via ERK/MAPK Activation. Neoplasia (New York, N.Y.), 20(9), 951–963.

• “The main drawback from my current RNA isolation protocol is the numerous centrifugation steps and the relatively low volume of RNA yield in the end. Life Magnetics provides a higher yield of RNA from samples and eliminates the use of centrifugation steps.”
  – Kendra Royston, Ph.D. at University of Chicago
• “Life Magnetics has a new technology, which is worth trying and their biggest advantage is skipping DNase step.”
  – Quan Taihao, Ph.D. and Qin Zhaoping at University of Michigan, Dermatology Department
• “It is easy to use and gives high quality and a greater yield at a much-reduced price. Also, the stability of RNA with the beads at room temperature before the final extraction step is a big plus.”
  – Sabita Saldanha, Ph.D. at Alabama State University
• “This system is significantly easier than Trizol because it can be done on the bench top, rather than a fume hood. It is of comparable ease to Qiagen systems. A potential advantage that this kit has over a Qiagen system is that it can be scaled more easily.”
  – Joe Endicott, Ph.D. at University of Michigan, School of Medicine Ann Arbor 
• “With Life Magnetics Carbon-Based RNA extraction kit, it was nice to switch things up and try a different protocol. The extraction method generated great RNA purity and yield.”
  – Kayla Lewis, Ph.D. at the University of Alabama at Birmingham
• “When I used the Life Magnetics IsoMag Carbon Based RNA purification kit, I was surprised how easy protocol was to follow and how much more RNA was extracted. The major advantage of this kit over other commercial kits is high RNA yield with no additional DNase cleanup steps.”
  – Seema Shah, Ph.D. at Wayne State University

• Lysis buffer
• Carbon beads
• Wash buffer